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The stress specific impact of ALKBH1 on tRNA cleavage and tiRNA generation 
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Impact of <t>Alkbh1</t> knockdown on cell fate and tRNA cleavage after stress. A: Annexin V FACS analysis (left graph) and SYBR gold staining following Alkbh1 KD and arsenite stress (400 µM for 4 hours). Alkbh1 KD reduced the levels of apoptotic and necrotic cells and rescued tRNA from cleavage. B: Annexin V FACS analysis and SYBR gold staining following Alkbh1 KD and antimycin stress (150 µg/ml for 4 hours). Alkbh1 KD had no impact on cell fate or tRNA cleavage following antimycin stress. (Note: FACS for As and antimycin stresses were performed simultaneously but the results presented in 2 graphs for easier comprehension and data presentation. FACS data were presented as ratio to unstressed (control) cells). C: Annexin V FACS analysis and SYBR gold staining following Alkbh1 KD and OGD-R stress. Alkbh1 KD also had no impact on cell death ratio following OGD-R and no apparent impact on tRNA cleavage. Asterisk: p < 0.05, N.S: not significant statistically. SYBR gold staining was performed with 500ng total RNA per lane.
The stress specific impact of ALKBH1 on tRNA cleavage and tiRNA generation RNA Biology, 2020 Jun 21
"Impact of <t>Alkbh1</t> knockdown on cell fate and tRNA cleavage after stress. A: Annexin V FACS analysis (left graph) and SYBR gold staining following Alkbh1 KD and arsenite stress (400 µM for 4 hours). Alkbh1 KD reduced the levels of apoptotic and necrotic cells and rescued tRNA from cleavage. B: Annexin V FACS analysis and SYBR gold staining following Alkbh1 KD and antimycin stress (150 µg/ml for 4 hours). Alkbh1 KD had no impact on cell fate or tRNA cleavage following antimycin stress. (Note: FACS for As and antimycin stresses were performed simultaneously but the results presented in 2 graphs for easier comprehension and data presentation. FACS data were presented as ratio to unstressed (control) cells). C: Annexin V FACS analysis and SYBR gold staining following Alkbh1 KD and OGD-R stress. Alkbh1 KD also had no impact on cell death ratio following OGD-R and no apparent impact on tRNA cleavage. Asterisk: p < 0.05, N.S: not significant statistically. SYBR gold staining was performed with 500ng total RNA per lane. "
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